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KMID : 0191119910060030246
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Journal of Korean Medical Science
1991 Volume.6 No. 3 p.246 ~ p.250
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Effect of glucose on the expression of c-myc gene in cultured RINm5F cell
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Kim SW
Yang IM/Kim JW/Kim YS/Kim KW/Choi YK/Suh KS
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Abstract
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The study was designed to examine the effect of glucose on the expression of c-myc gene in cultured RINm5F cells. After monolayer culture was established in RPMI 1640 media supplemented with 10% fetal calf serum (FCS), the cells were cultured in various concentrations of glucose and 1 or 10% FCS for another 24 hours. A mRNA was extracted from the cultured cells by a single step method, and Northern analysis was done to detect RNA band. A 0.5 kilobase single band was detected as c-myc mRNA. The expression of c-myc gene mRNA was reduced with increased concentration of glucose with 1% FCS. However, supplementation of 10% FCS abolished the effect of glucose on expression of c-myc gene. These findings suggested that glucose in conjunction with other growth promoting factors played an important role in expression of oncogene and cell growth in RINm5F cells.
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KEYWORD
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